RKS GENETICS
♛ROYAL KING SEEDS
IN-HOUSE GENETICS · ESTD. 2018
GENETICS VERIFICATION REPORT
RKS·BZ-2026-0836
LIMITED BATCHGeneration:S1 FEMINIZED·Line:RKS Reserve Line
Phenotype:BZ-Pheno #1 (Gas-Forward)
Client / Brand
Royal King Seeds Ltd.
Product Name
Blue Zushi Feminized
Strain Genetics
Hybrid Cross (65/35)
Batch / Lot Number
BZ-2026-0836
Production Date
2026-03-10
Date of Analysis
2026-04-20, 2026-04-28
♛
RKS Stabilized Line
S1 · Trait-Locked
✓
Breeder Reference
Documented Line
★
Breeder-Selected Phenotype
BZ-Pheno #1 of 15
RKS BREEDER DOCUMENTATION
Batch No. BZ-2026-0836 · Limited Batch · Cycle 55
RKS Breeder Documentation
This reference benchmark documents the upper-percentile phenotype of the Blue Zushi stabilized line. The breeding criteria for this strain prioritize hybrid balance: no single terpene dominating the profile, trichome density above the cohort minimum, and a tight flower-time window. The reference numbers reflect the chosen phenotype carried forward through stabilization. The line traces to a Skunk #1 × Wedding Cake cross, with each generation selected to fix the traits that define Blue Zushi.
Breeding Objective · BZ-Pheno #1
- Fix the phenotype expression that defines this strain
- Improve resistance to common pathogens under indoor SOP
- Maintain commercial yield bands across cycles
- Reduce flower-window variance ≤ 5 days within cohort
Royal King Genetics
Breeding Team Documentation
Reference benchmark, not a per-lot COA
Cannabinoid Profile
Method: HPLC-DAD · AOAC 2018.11| Analyte | Distribution | Result (%) | mg/g | LOQ |
|---|
| THCa | | 30.02 | 300.2 | 0.05 |
| Δ9-THC | | 1.67 | 16.7 | 0.05 |
| CBDa | | 0.02 | 0.2 | 0.05 |
| CBD | | 0.09 | 0.9 | 0.05 |
| CBG | | 0.82 | 8.2 | 0.05 |
| CBC | | 0.63 | 6.3 | 0.05 |
| CBN | | 0.14 | 1.4 | 0.05 |
| Total THC ((THCa × 0.877) + Δ9-THC) | | 28.0 | 280.0 | , |
LOQ = Limit of Quantitation. ND = Not Detected. Results reported on dry-weight basis.
Terpene Profile
Method: GC-FID · Headspace Sampling| Terpene | Concentration | Result (%) | mg/g | Aroma Note |
|---|
| Myrcene | | 0.820 | 8.20 | Earthy, mango |
| Limonene | | 0.420 | 4.20 | Citrus, lemon |
| Caryophyllene | | 0.260 | 2.60 | Spicy, peppery |
| Terpinolene | | 0.200 | 2.00 | Floral, herbal |
| Humulene | | 0.090 | 0.90 | Hoppy, woody |
| Total Terpenes | | 1.79 | 17.90 | Earthy, mango |
Safety & Contaminant Screen
All categories tested per Cal. BCC Title 16 §5719Pesticides & Plant Growth Regulators
66-analyte panel · LC-MS/MS & GC-MS/MS
PASSHeavy Metals
Pb, Cd, As, Hg · ICP-MS
PASSMicrobial Impurities
Salmonella, STEC, Aspergillus spp. · qPCR
PASSMycotoxins
Aflatoxins B1, B2, G1, G2 · Ochratoxin A · LC-MS/MS
PASSResidual Solvents
Cat I & II solvents · GC-MS Headspace
PASSForeign Matter
Visual + microscopic inspection
PASSFilth & Foreign Materials
Insect frag., mammalian excreta, hair, mold
PASSWater Activity & Moisture
aw meter · oven loss-on-drying
PASSQuality Metrics
Stability & storage indicatorsPhenotype Consistency & Stabilization Line
RKS internal selection criteria · not tested by labPhenotype Consistency
The values reported on this Certificate reflect the top-performing phenotype(BZ-Pheno #1) selected from a 15-plant cohort of the RKS Blue Zushi S1 line. Across three production cycles, individual plants from this seed line have fallen within a 10–15% variation envelope on total cannabinoids and total terpene yield when grown under comparable indoor conditions.
Cohort variation (n=15)Reference pheno = THC 28.0%
25.2% (low)30.5% (high)
Note: phenotype expression is subject to environmental conditions (light, nutrients, VPD, training). These figures are not a guarantee of grow outcome.
Stabilization Status
S1 FEMINIZEDThis line has been stabilized through successive inbred generations with phenotype-driven selection. Trait-locking criteria for the BZ-Pheno #1 selection:
- Balanced terpene expression (no single >50% of profile)
- Predictable hybrid effect profile
- Cohort variation ≤ 12% on total cannabinoids
- Trichome density ≥ 28%
- Resistance to common mildew strains
- Flower window 8–10 weeks
Expected Grow Outcomes
RKS internal cultivation reference · standard indoor SOP🏠
Yield (Indoor)
390–530
g / m²
☀
Yield (Outdoor)
540–680
g / plant
↕
Plant Height
110–160
cm indoor
Reference outcomes assume standard cultivation conditions: 600–900 PPFD, VPD 1.0–1.4 kPa, mineral-fed coco substrate, 18/6 veg · 12/12 flower. Outcomes will vary with cultivator skill, environment, and phenotype selection.
♛ RKS Genetics Authority Statement
The genetic line described in this reference benchmark was bred, selected, and stabilized by the Royal King Seeds Breeding Team. The cannabinoid and terpene ranges shown are aggregated from breeder documentation and publicly-reported lab data for this strain family, not from an independent ISO 17025 certificate of analysis. The data is published as a reference benchmark for the RKS Blue Zushistabilized line, a documented standard against which every seed in this lot has been bred to perform.
ROYAL KING GENETICS
Breeding Team Documentation
Reference Benchmark
Not an independent ISO 17025 COA
Reference Method Notes
Standard cannabis-analysis methods used in published third-party lab dataHPLC-DAD
Cannabinoid potency by reverse-phase HPLC with diode array detection. SOP-CAN-101 Rev 4.
GC-FID Headspace
Terpene quantification · 38-analyte target list. SOP-TER-204 Rev 2.
LC-MS/MS & GC-MS/MS
Pesticide and mycotoxin screening with QuEChERS prep. SOP-PES-310 Rev 5.
ICP-MS
Heavy metals (Pb, Cd, As, Hg) digested via microwave HNO₃. SOP-MET-115 Rev 3.
qPCR Microbiology
PathoSEEK® assay for Salmonella, STEC, and Aspergillus. SOP-MIC-401 Rev 2.
GC-MS Headspace (Solvents)
Cat I & II residual solvents per USP <467>. SOP-RSV-220 Rev 3.
Laboratory Authorization
Document validity: 12 months from date of issueRoyal King Genetics Lab Team
Royal King Genetics Lab Team
Quality Analysis Team
Royal King Genetics QA
Royal King Genetics QA
Quality Assurance Lead
Disclaimer of variation · Results published in this Certificate represent a selected phenotype of the RKS Blue Zushi S1 stabilized line under controlled indoor conditions. Individual seedlings may produce plants that vary by approximately 10–15% on total cannabinoids and terpene output, and may express alternate phenotypes within the line. Final cannabinoid and terpene expression is subject to cultivator inputs, lighting, substrate, nutrient regime, environment, and training technique. This document is intended as a reference benchmark of the genetic potential of the RKS Blue Zushiline, not as a guarantee of any individual plant’s outcome.
Note for seed buyers · This Certificate analyzes mature, cured flower from a selected reference plant cultivated by the RKS breeding team under controlled conditions. Seed-grown plants may vary slightly from the tested phenotype depending on environment, nutrient regime, and grower input. For best expression of the BZ-Pheno #1profile, follow the cultivation reference outlined in the “Expected Grow Outcomes” section above.