Product Name
Do-Si-Dos Auto
RKS GENETICS
CANNALYTICS LABORATORIES, INC.
Independent Third-Party Verification Lab
2440 Industrial Pkwy West · Hayward, CA 94545
Tel: (510) 555-2240 · lab@cannalytics-coa.com
License #: C8-0000142-LIC
CERTIFICATE OF ANALYSIS
· GENETICS PROFILE ·
DOC ID: CAN-2026-04-D-0801
Lab verify
cannalytics·coa
cannalytics·coa
RKS genetics
royalkingseeds.us
royalkingseeds.us
CORE LINEGeneration:AUTO F4·Line:RKS Master Selection
Phenotype:D-Pheno #2 (Gas-Forward)
Client / Brand
Royal King Seeds Ltd.
Strain Genetics
Autoflower Hybrid (50/50)
Sample ID
RKS-D-AUTO-0801
Batch / Lot Number
D-2026-0801
Production Date
2026-03-17
Date Received
2026-04-13
Date of Analysis
2026-04-20 — 2026-04-27
RKS Stabilized Line
Auto · Trait-Locked
Verified Genetics
ISO 17025 Co-Signed
Breeder-Selected Phenotype
D-Pheno #2 of 12
RKS BREEDER NOTES
Batch No. D-2026-0801 · Core Line · Cycle 50
From the RKS Breeding Floor
The Do-Si-Dos line has been stabilized through successive inbred generations with phenotype-driven selection. The hybrid character — predictable balance, terpene complexity, manageable structure — has been our breeding objective from cycle one. The phenotype reported here is the top-performing selection from our most recent twelve-plant cohort; the variation envelope on the report shows the realistic spread you can expect from seeds in this line. The line traces to a Chemdawg × Skunk #1 cross, with each generation selected to fix the traits that define Do-Si-Dos.
Breeding Objective · D-Pheno #2
- Intensify the dominant terpene expression of the line
- Reduce structural variation across cohort
- Improve mold & powdery-mildew resistance under high-humidity flower
- Lock the flowering window with ≤ 7 day cohort variance
D. Van Royen
Master Breeder · Royal King Seeds
Genetics verified by RKS Breeding Team
Genetics verified by RKS Breeding Team
Total THC
21.8%
Total CBD
0.24%
Total Terpenes
1.90%
Safety
ALL PASS
Cannabinoid Profile
| Analyte | Distribution | Result (%) | mg/g | LOQ |
|---|---|---|---|---|
| THCa | 23.72 | 237.2 | 0.05 | |
| Δ9-THC | 1.00 | 10.0 | 0.05 | |
| CBDa | 0.20 | 2.0 | 0.05 | |
| CBD | 0.06 | 0.6 | 0.05 | |
| CBG | 0.47 | 4.7 | 0.05 | |
| CBC | 0.38 | 3.8 | 0.05 | |
| CBN | 0.19 | 1.9 | 0.05 | |
| Total THC ((THCa × 0.877) + Δ9-THC) | 21.8 | 218.0 | — |
LOQ = Limit of Quantitation. ND = Not Detected. Results reported on dry-weight basis.
Terpene Profile
| Terpene | Concentration | Result (%) | mg/g | Aroma Note |
|---|---|---|---|---|
| Myrcene | 0.710 | 7.10 | Earthy, mango | |
| Limonene | 0.510 | 5.10 | Citrus, lemon | |
| Caryophyllene | 0.370 | 3.70 | Spicy, peppery | |
| Camphene | 0.250 | 2.50 | Damp woodland | |
| Humulene | 0.060 | 0.60 | Hoppy, woody | |
| Total Terpenes | 1.90 | 19.00 | Earthy, mango |
Safety & Contaminant Screen
Pesticides & Plant Growth Regulators
66-analyte panel · LC-MS/MS & GC-MS/MS
Heavy Metals
Pb, Cd, As, Hg · ICP-MS
Microbial Impurities
Salmonella, STEC, Aspergillus spp. · qPCR
Mycotoxins
Aflatoxins B1, B2, G1, G2 · Ochratoxin A · LC-MS/MS
Residual Solvents
Cat I & II solvents · GC-MS Headspace
Foreign Matter
Visual + microscopic inspection
Filth & Foreign Materials
Insect frag., mammalian excreta, hair, mold
Water Activity & Moisture
aw meter · oven loss-on-drying
Quality Metrics
Moisture Content
12.4%
Water Activity (aw)
0.55
Density (g/mL)
0.43
Phenotype Consistency & Stabilization Line
Phenotype Consistency
The values reported on this Certificate reflect the top-performing phenotype(D-Pheno #2) selected from a 12-plant cohort of the RKS Do-Si-Dos Auto line. Across three production cycles, individual plants from this seed line have fallen within a 10–15% variation envelope on total cannabinoids and total terpene yield when grown under comparable indoor conditions.
Note: phenotype expression is subject to environmental conditions (light, nutrients, VPD, training). These figures are not a guarantee of grow outcome.
Stabilization Status
AUTO F4This line has been stabilized through successive inbred generations with phenotype-driven selection. Trait-locking criteria for the D-Pheno #2 selection:
- Cookies-family aroma fidelity
- Caryophyllene > 0.35%
- Trait stability across 3 production cycles
- Bud density 0.38–0.45 g/mL
- Mid-night-temperature tolerance
- Cohort flower variance ≤ 6 days
Expected Grow Outcomes
Flower Time
9 – 11
weeks
Yield (Indoor)
251–352
g / m²
Yield (Outdoor)
81–182
g / plant
Plant Height
61–102
cm total
Difficulty
Easy
beginner+
Reference outcomes are based on RKS breeding-room conditions: 600–900 PPFD, VPD 1.0–1.4 kPa, mineral-fed coco substrate, 18/6 veg · 12/12 flower. Outcomes will vary with cultivator skill, environment, and phenotype selection.
Analytical Methods
HPLC-DAD
Cannabinoid potency by reverse-phase HPLC with diode array detection. SOP-CAN-101 Rev 4.
GC-FID Headspace
Terpene quantification · 38-analyte target list. SOP-TER-204 Rev 2.
LC-MS/MS & GC-MS/MS
Pesticide and mycotoxin screening with QuEChERS prep. SOP-PES-310 Rev 5.
ICP-MS
Heavy metals (Pb, Cd, As, Hg) digested via microwave HNO₃. SOP-MET-115 Rev 3.
qPCR Microbiology
PathoSEEK® assay for Salmonella, STEC, and Aspergillus. SOP-MIC-401 Rev 2.
GC-MS Headspace (Solvents)
Cat I & II residual solvents per USP <467>. SOP-RSV-220 Rev 3.
Laboratory Authorization
Dr. Marcus Reyes
Dr. Marcus Reyes, Ph.D.
Lead Analyst · Cannabinoid & Terpene Chemistry
Mark Linden
Mark Linden
Laboratory Director · ISO 17025 Approving Officer
Disclaimer of variation · Results published in this Certificate represent a selected phenotype of the RKS Do-Si-Dos Auto stabilized line under controlled indoor conditions. Individual seedlings may produce plants that vary by approximately 10–15% on total cannabinoids and terpene output, and may express alternate phenotypes within the line. Final cannabinoid and terpene expression is subject to cultivator inputs — lighting, substrate, nutrient regime, environment, and training technique. This document is intended as a reference benchmark of the genetic potential of the RKS Do-Si-Dos line, not as a guarantee of any individual plant’s outcome.
Note for seed buyers · This Certificate analyzes mature, cured flower from a selected reference plant cultivated by the RKS breeding team under controlled conditions. Seed-grown plants may vary slightly from the tested phenotype depending on environment, nutrient regime, and grower input. For best expression of the D-Pheno #2 profile, follow the cultivation reference outlined in the “Expected Grow Outcomes” section above.
Note for seed buyers · This Certificate analyzes mature, cured flower from a selected reference plant cultivated by the RKS breeding team under controlled conditions. Seed-grown plants may vary slightly from the tested phenotype depending on environment, nutrient regime, and grower input. For best expression of the D-Pheno #2 profile, follow the cultivation reference outlined in the “Expected Grow Outcomes” section above.